Coenzyme Binding to L-α-Glycerophosphate Dehydrogenase
نویسندگان
چکیده
منابع مشابه
L-3-glycerophosphate dehydrogenase from pig brain mitochondria.
1. A method is described for extraction and partial purification of mitochondrial l-3-glycerophosphate dehydrogenase from pig brain. 2. By the criteria that have so far been applied, the extraction and purification procedures do not modify the activity of the enzyme towards artificial electron acceptors. 3. The amounts of acid-liberatable flavine and iron in the preparation were measured. 4. A ...
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L-3-Hydroxyacyl coenzyme A dehydrogenase (L-3hydroxyacyl-CoA:NAD oxidoreductase; EC 1 .l .1.35) is a mitochondrial enzyme involved in the beta oxidation of fatty acids. The enzyme, as isolated from pig heart muscle, hasM, 67 000 and contains two identical subunits [l]. The amino acid sequence has recently been completed and appears to have little homology with other dehydrogenases [2]. Like man...
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Among the many methods which are available for studying the binding of substances to proteins (l), those applied most commonly for pyridine nucleotide-binding to dehydrogenases have been the spectrophotometric (2) and ultracentrifuge measurements (3). An additional method has been introduced through the observations of Boyer and Theorell (4) who found that reduced diphosphopyridine nucleotide i...
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Enzymatic activity was determined in an optical test, based on the measurement of reduced DPN at 340 rnp in the Beckman spectrophotometer. The reaction was carried out in phosphate or cysteine buffer at pH 7. Synthetic dihydroxyacetone phosphate (5) with reduced DPN or racemic cr-glycerophosphate with DPN was used as substrate to measure the forward and backward reactions, respectively. The con...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1969
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)91794-1